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ms2 p65 hsf1 activator expression construct  (Addgene inc)


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    Structured Review

    Addgene inc ms2 p65 hsf1 activator expression construct
    Ms2 P65 Hsf1 Activator Expression Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ms2+p65+hsf1+activator+expression+construct/MS2-P65-HSF1_GFP+(Plasmid+%2361423)/pmc06417287-240-20-25
    Average 94 stars, based on 79 article reviews
    ms2 p65 hsf1 activator expression construct - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
    Article Snippet: To test if dCas9-SAM could activate endogenous target genes when recruited to the sites where we added the GBSs, we created gRNAs containing MS2 loops by cloning the target sequence ( ) into the sgRNA(MS2) plasmid (#61424; Addgene). .. Next, U2OS-GR cells were transfected with 600 ng each of the MS2-containing gRNA, dCas9-VP64 expression construct (#48223; Addgene), and an MS2-p65-HSF1 activator expression construct (#61423; Addgene) by nucleofection (Lonza Nucleofector kit V) according to the manufacturer’s instructions. .. 24 h after transfection, total RNA was isolated using the RNeasy kit (QIAGEN), DNase-I digested and reverse transcribed using random primers (NEB) and analyzed by qPCR using primers listed in .

    Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
    Article Snippet: To test if dCas9-SAM could activate endogenous target genes when recruited to the sites where we added the GBSs, we created gRNAs containing MS2 loops by cloning the target sequence ( ) into the sgRNA(MS2) plasmid (Addgene #61424). .. Next, U2OS-GR cells were transfected with 600 ng each of the MS2-containing gRNA, dCas9-VP64 expression construct (Addgene #48223) and a MS2-p65-HSF1 activator expression construct (Addgene #61423) by nucleofection (Lonza Nucleofector Kit V) according to the manufacturer’s instructions. .. 24h after transfection, total RNA was isolated using the RNeasy kit (Qiagen), DNase-I digested and reverse transcribed using random primers (NEB) and analyzed by qPCR using primers listed in .

    Expressing:

    Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
    Article Snippet: To test if dCas9-SAM could activate endogenous target genes when recruited to the sites where we added the GBSs, we created gRNAs containing MS2 loops by cloning the target sequence ( ) into the sgRNA(MS2) plasmid (#61424; Addgene). .. Next, U2OS-GR cells were transfected with 600 ng each of the MS2-containing gRNA, dCas9-VP64 expression construct (#48223; Addgene), and an MS2-p65-HSF1 activator expression construct (#61423; Addgene) by nucleofection (Lonza Nucleofector kit V) according to the manufacturer’s instructions. .. 24 h after transfection, total RNA was isolated using the RNeasy kit (QIAGEN), DNase-I digested and reverse transcribed using random primers (NEB) and analyzed by qPCR using primers listed in .

    Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
    Article Snippet: To test if dCas9-SAM could activate endogenous target genes when recruited to the sites where we added the GBSs, we created gRNAs containing MS2 loops by cloning the target sequence ( ) into the sgRNA(MS2) plasmid (Addgene #61424). .. Next, U2OS-GR cells were transfected with 600 ng each of the MS2-containing gRNA, dCas9-VP64 expression construct (Addgene #48223) and a MS2-p65-HSF1 activator expression construct (Addgene #61423) by nucleofection (Lonza Nucleofector Kit V) according to the manufacturer’s instructions. .. 24h after transfection, total RNA was isolated using the RNeasy kit (Qiagen), DNase-I digested and reverse transcribed using random primers (NEB) and analyzed by qPCR using primers listed in .

    Construct:

    Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
    Article Snippet: To test if dCas9-SAM could activate endogenous target genes when recruited to the sites where we added the GBSs, we created gRNAs containing MS2 loops by cloning the target sequence ( ) into the sgRNA(MS2) plasmid (#61424; Addgene). .. Next, U2OS-GR cells were transfected with 600 ng each of the MS2-containing gRNA, dCas9-VP64 expression construct (#48223; Addgene), and an MS2-p65-HSF1 activator expression construct (#61423; Addgene) by nucleofection (Lonza Nucleofector kit V) according to the manufacturer’s instructions. .. 24 h after transfection, total RNA was isolated using the RNeasy kit (QIAGEN), DNase-I digested and reverse transcribed using random primers (NEB) and analyzed by qPCR using primers listed in .

    Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
    Article Snippet: To test if dCas9-SAM could activate endogenous target genes when recruited to the sites where we added the GBSs, we created gRNAs containing MS2 loops by cloning the target sequence ( ) into the sgRNA(MS2) plasmid (Addgene #61424). .. Next, U2OS-GR cells were transfected with 600 ng each of the MS2-containing gRNA, dCas9-VP64 expression construct (Addgene #48223) and a MS2-p65-HSF1 activator expression construct (Addgene #61423) by nucleofection (Lonza Nucleofector Kit V) according to the manufacturer’s instructions. .. 24h after transfection, total RNA was isolated using the RNeasy kit (Qiagen), DNase-I digested and reverse transcribed using random primers (NEB) and analyzed by qPCR using primers listed in .



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    Addgene inc ms2 p65 hsf1 activator expression construct
    Ms2 P65 Hsf1 Activator Expression Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ms2+p65+hsf1+activator+expression+construct/MS2-P65-HSF1_GFP+(Plasmid+%2361423)/pmc06417287-240-20-25
    Average 94 stars, based on 1 article reviews
    ms2 p65 hsf1 activator expression construct - by Bioz Stars, 2026-09
    94/100 stars
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